Recombinant Vaccinia virus DNA topoisomerase 1B (OPG111), Vaccinia virus (strain Copenhagen) (VACV), E.coli, N-terminal 6xHis-tagged, 40.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Vaccinia virus DNA topoisomerase 1B (OPG111)

Recombinant Vaccinia virus DNA topoisomerase 1B (OPG111) — Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Purity >90%.

SKU: BCRECP-003955 Categories: , ,
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Product Specifications

Product SkuBCRECP-003955
Product DescriptionRecombinant Vaccinia virus DNA topoisomerase 1B (OPG111) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-314aa. [Accession | P68697].
Uniprot No.P68697
Gene NamesOPG111
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-314aa
SpeciesVaccinia virus (strain Copenhagen) (VACV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight40.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.

Biological Context

Subcellular Location: Virion

Product Specifications

Recombinant Vaccinia virus DNA topoisomerase 1B (OPG111) is a recombinant protein from Vaccinia virus (strain Copenhagen) (VACV), expressed in E.coli, covering amino acids 1-314aa, with N-terminal 6xHis-tagged tag, molecular weight 40.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone

Subcellular Location

Virion

Gene: OPG111  |  Organism: Vaccinia virus (strain Copenhagen)  |  Synonyms: DNA topoisomerase I; Late protein H6

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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