Recombinant Vaccinia virus Envelope phospholipase F13 (VACWR052), Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 49.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Vaccinia virus Envelope phospholipase F13 (VACWR052)

Recombinant Vaccinia virus Envelope phospholipase F13 (VACWR052) — Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell. Purity >90%.

SKU: BCRECP-004706 Categories: ,
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Product Specifications

Product SkuBCRECP-004706
Product DescriptionRecombinant Vaccinia virus Envelope phospholipase F13 (VACWR052) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-372aa. [Accession | P04021].
Uniprot No.P04021
Gene NamesVACWR052
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-372aa
SpeciesVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight49.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell. Produces the wrapped form of virus that is required for cell-to-cell spread. Acts as a lipase with broad specificity including phospholipase C, phospholipase A, and triacylglycerol lipase activities.

Product Specifications

Recombinant Vaccinia virus Envelope phospholipase F13 (VACWR052) is a recombinant protein, expressed in E.coli, covering amino acids 1-372aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 49.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell (PubMed:17475658, PubMed:27466413, PubMed:8999886). Produces the wrapped form of virus that is required for cell-to-cell spread (PubMed:27466413, PubMed:29540596). Acts as a lipase with broad specificity including phospholipase C, phospholipase A, and triacylglycerol lipase activities (PubMed:9405398)

Gene: OPG057  |  Organism: Vaccinia virus (strain Western Reserve)

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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