Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial, Vaccinia virus (strain Copenhagen) (VACV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial

Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial — Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). Purity >90%.

SKU: BCRECP-002502 Categories: ,
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Product Specifications

Product SkuBCRECP-002502
Product DescriptionRecombinant Vaccinia virus Scaffold protein D13 (D13L) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-230aa. [Accession | P68441].
Uniprot No.P68441
Gene NamesD13L
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-230aa
SpeciesVaccinia virus (strain Copenhagen) (VACV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis.

Biological Context

Subcellular Location: Membrane (Peripheral membrane protein)

Product Specifications

Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial is a recombinant protein from Vaccinia virus (strain Copenhagen) (VACV), expressed in E.coli, covering amino acids 1-230aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of OPG158 N-terminus

Subcellular Location

Membrane (Peripheral membrane protein)

Subunit

Homotrimer. Self-assembles to form a layer. Interacts with OPG158 (via N-terminus); this interaction is necessary for OPG125 association with membranes

Gene: OPG125  |  Organism: Vaccinia virus (strain Copenhagen)  |  Synonyms: 62 kDa protein; Rifampicin resistance protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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