Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial, Vaccinia virus (strain Copenhagen) (VACV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial

Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial — Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). Purity >90%.

SKU: BCRECP-002502 Categories: ,
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Product Specifications

Product SkuBCRECP-002502
Product DescriptionRecombinant Vaccinia virus Scaffold protein D13 (D13L) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-230aa. [Accession | P68441].
Uniprot No.P68441
Gene NamesD13L
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-230aa
SpeciesVaccinia virus (strain Copenhagen) (VACV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis.

Biological Context

Subcellular Location: Membrane (Peripheral membrane protein)

Product Specifications

Recombinant Vaccinia virus Scaffold protein D13 (D13L), partial is a recombinant protein from Vaccinia virus (strain Copenhagen) (VACV), expressed in E.coli, covering amino acids 1-230aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of OPG158 N-terminus

Subcellular Location

Membrane (Peripheral membrane protein)

Subunit

Homotrimer. Self-assembles to form a layer. Interacts with OPG158 (via N-terminus); this interaction is necessary for OPG125 association with membranes

Gene: OPG125  |  Organism: Vaccinia virus (strain Copenhagen)  |  Synonyms: 62 kDa protein; Rifampicin resistance protein

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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