Recombinant Varicella-zoster virus Envelope glycoprotein B (gB), partial, Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3), E.coli, N-terminal 6xHis-tagged, 42.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Varicella-zoster virus Envelope glycoprotein B (gB), partial

Recombinant Varicella-zoster virus Envelope glycoprotein B (gB), partial — Envelope glycoprotein that forms spikes at the surface of virion envelope. Purity >85%.

SKU: BCRECP-002826 Categories: ,
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Product Specifications

Product SkuBCRECP-002826
Product DescriptionRecombinant Varicella-zoster virus Envelope glycoprotein B (gB) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 156-471aa. [Accession | P09257].
Uniprot No.P09257
Gene NamesgB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region156-471aa
SpeciesVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Tag InfoN-terminal 6xHis-tagged
Molecular weight42.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein); Host Golgi apparatus membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Varicella-zoster virus Envelope glycoprotein B (gB), partial is a recombinant protein from Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3), expressed in E.coli, covering amino acids 156-471aa, with N-terminal 6xHis-tagged tag, molecular weight 42.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein); Host Golgi apparatus membrane (Single-pass type I membrane protein)

Subunit

Homotrimer; disulfide-linked. Binds to heparan sulfate proteoglycans. Interacts with gH/gL heterodimer (By similarity). Interacts with gE

Gene: gB  |  Organism: Varicella-zoster virus (strain Dumas)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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