Recombinant Escherichia coli Translational regulator CsrA (csrA), Escherichia coli (strain K12 / DH10B), E.coli, Tag-Free, 6.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Translational regulator CsrA (csrA)

Recombinant Escherichia coli Translational regulator CsrA (csrA) — A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Purity >85%.

SKU: BCRECP-002463 Category:
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Product Specifications

Product SkuBCRECP-002463
Product DescriptionRecombinant Escherichia coli Translational regulator CsrA (csrA) Protein is expressed from E.coli with Tag-Free. It contains 1-61aa. [Accession | B1XCM4].
Uniprot No.B1XCM4
Gene NamescsrA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-61aa
SpeciesEscherichia coli (strain K12 / DH10B)
Tag InfoTag-Free
Molecular weight6.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli Translational regulator CsrA (csrA) is a recombinant protein from Escherichia coli (strain K12 / DH10B), expressed in E.coli, covering amino acids 1-61aa, with Tag-Free tag, molecular weight 6.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA. Its function is antagonized by small RNA(s)

Subcellular Location

Cytoplasm

Subunit

Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core, while the alpha-helices form wings that extend away from the core

Gene: csrA  |  Organism: Escherichia coli (strain K12 / DH10B)  |  Synonyms: Carbon storage regulator

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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