Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1), Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 44.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)

Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) — Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Purity >85%.

SKU: BCRECP-002817 Categories: ,
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Product Specifications

Product SkuBCRECP-002817
Product DescriptionRecombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-338aa. [Accession | P25858].
Uniprot No.P25858
Gene NamesGAPC1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-338aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight44.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Expressed in leaves, stems and siliques and at lower levels in roots and flowers
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Product Specifications

Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 1-338aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 44.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility (PubMed:18820081). Involved in response to oxidative stress by mediating plant responses to abscisic acid (ABA) and water deficits through the activation of PLDDELTA and production of phosphatidic acid (PA), a multifunctional stress signaling lipid in plants (PubMed:22589465). Associates with FBA6 to the outer mitochondrial membrane, in a redox-dependent manner, leading to binding and bundling of actin. Actin binding and bundling occurs under oxidizing conditions and is reversible under reducing conditions. May be part of a redox-dependent retrograde signal transduction network for adaptation upon oxidative stress (PubMed:23316205). Binds DNA in vitro. Together with DNA polymerase II subunit B3-1 (DPB3-1) and GAPC2, enhances heat tolerance and promotes the expression of heat-inducible genes (PubMed:32651385)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Expressed in leaves, stems and siliques and at lower levels in roots and flowers

Subunit

Homotetramer (By similarity). Interacts with PLDDELTA (PubMed:22589465). Interacts with FBA6 and VDAC3 (PubMed:23316205). Binds to DPB3-1/NF-YC10 in response to heat-stress; this interaction promotes DPB3-1/NF-YC10 DNA-binding ability to its target promoter (PubMed:32651385)

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Gene: GAPC1  |  Organism: Arabidopsis thaliana  |  Synonyms: NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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