Recombinant Rat FAD-linked sulfhydryl oxidase ALR (Gfer), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-SUMO-tagged, 38.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rat FAD-linked sulfhydryl oxidase ALR (Gfer)

Recombinant Rat FAD-linked sulfhydryl oxidase ALR (Gfer) — FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial inter… Purity >90%.

SKU: BCRECP-002842 Categories: ,
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Product Specifications

Product SkuBCRECP-002842
Product DescriptionRecombinant Rat FAD-linked sulfhydryl oxidase ALR (Gfer) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-198aa. [Accession | Q63042].
Uniprot No.Q63042
Gene NamesGfer
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-198aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight38.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.

Biological Context

Subcellular Location: Mitochondrion intermembrane space; Mitochondrion

Product Specifications

Recombinant Rat FAD-linked sulfhydryl oxidase ALR (Gfer) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 1-198aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 38.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis

Subcellular Location

Mitochondrion intermembrane space; Mitochondrion

Subunit

Homodimer; disulfide-linked (PubMed:12717032, PubMed:22948913). Interacts with CHCHD4/MIA40 (By similarity)

Gene: Gfer  |  Organism: Rattus norvegicus  |  Synonyms: Augmenter of liver regeneration
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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