Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active), Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.0 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active)

Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active) — recombinant protein from Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000033 Categories: , , ,
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Product SKUBCACP-000033
Product DescriptionRecombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 306-527aa. [Accession | P59594].
Uniprot No.P59594
Gene NamesS
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region306-527aa
SpeciesHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 , the EC50 is 5.056-7.559 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 7.941-10.49 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(S glycoprotein)(E2)(Peplomer protein)(Spike protein S1)(Spike protein S2)

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active) is a recombinant protein from Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), expressed in Mammalian cell, covering amino acids 306-527aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity). Binds to human and palm civet ACE2 and human CLEC4M/DC-SIGNR. Interacts with the accessory proteins 3a and 7a

Gene: S  |  Organism: Severe acute respiratory syndrome coronavirus  |  Synonyms: E2; Peplomer protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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