Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active), Escherichia (strain K12), E.coli, N-terminal GST-tagged, 67.6 kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active)

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active) — Reductase involved in the detoxification of N-hydroxylated base analogs. Purity >85%.

SKU: BCACP-000042 Category:
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Product SKUBCACP-000042
Product DescriptionRecombinant strain K12 coli Uncharacterized protein YcbX (ycbX) protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-369aa. [Accession | P75863].
Uniprot No.P75863
Gene NamesycbX
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-369aa
SpeciesEscherichia (strain K12)
Tag InfoN-terminal GST-tagged
Molecular weight67.6 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ldcC at 5 μg/ml can bind Escherichia coli ycbX, the EC50 of Escherichia coli ycbX protein is 359.062 - 628.116 μg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesycbX; b0947; JW5126; Uncharacterized protein YcbX

Function

Reductase involved in the detoxification of N-hydroxylated base analogs. Contributes to the detoxification of 6-N-hydroxylaminopurine (HAP) by catalyzing the reduction of HAP to adenine. In the presence of methyl viologen as reduced electron donor, it is also active toward other N-hydroxy substrates such as 2-amino-6-hydroxylaminopurine (2-AHAP), N-hydroxyadenosine, N-hydroxyacetamidine, N-hydroxyurea and hydroxylamine, but it cannot use N-oxide and sulfoxide substrates, or nitrate and chlorate.

Product Specifications

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active) is a recombinant protein from Escherichia (strain K12), expressed in E.coli, covering amino acids 1-369aa, with N-terminal GST-tagged tag, molecular weight 67.6 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Reductase involved in the detoxification of N-hydroxylated base analogs (PubMed:18312271). Contributes to the detoxification of 6-N-hydroxylaminopurine (HAP) by catalyzing the reduction of HAP to adenine (PubMed:18312271, PubMed:35640667). In the presence of methyl viologen as reduced electron donor, it is also active toward other N-hydroxy substrates such as 2-amino-6-hydroxylaminopurine (2-AHAP), N-hydroxyadenosine, N-hydroxyacetamidine, N-hydroxyurea and hydroxylamine, but it cannot use N-oxide and sulfoxide substrates, or nitrate and chlorate (PubMed:35640667). The reducing equivalents required for the detoxification reaction may be provided by the sulfite reductase component CysJ, which functions as a partner of YcbX (PubMed:20118259). YcbX is not involved in molybdenum cofactor (MoCo) biosynthesis or in MoCo sulfuration (PubMed:18312271)

Gene: ycbX  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Hydroxylaminopurine resistance molybdoenzyme YcbX
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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