Recombinant Human Natural cytotoxicity triggering receptor 3 (NCR3), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 41.8 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.

Recombinant Human Natural cytotoxicity triggering receptor 3 (NCR3), partial (Active)

Recombinant Human Natural cytotoxicity triggering receptor 3 (NCR3), partial (Active) — Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Purity >95%.

SKU: BCACP-000392 Category:
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Product SKUBCACP-000392
Product DescriptionRecombinant Human Natural cytotoxicity triggering receptor 3 (NCR3) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 19-135aa. [Accession | O14931].
Uniprot No.O14931
Gene NamesNCR3
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region19-135aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight41.8 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human VTCN1 at 2 μg/mL can bind Anti-VTCN1 recombinant antibody . The EC50 is 9.352-10.38 ng/mL.;VTCN1 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human VTCN1 with an affinity constant of 16.1 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesNatural cytotoxicity triggering receptor 3; Activating natural killer receptor p30; Natural killer cell p30-related protein (NK-p30; NKp30); CD337; NCR3;1C7, LY117

Function

Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Selectively expressed by all resting and activated NK cells and weakly expressed in spleen

Product Specifications

Recombinant Human Natural cytotoxicity triggering receptor 3 (NCR3), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-135aa, with C-terminal hFc1-tagged tag, molecular weight 41.8 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Selectively expressed by all resting and activated NK cells and weakly expressed in spleen

Subunit

Homodimer in the unliganted form (PubMed:21422170, PubMed:21444796). Interacts with CD3Z (PubMed:10562324, Ref.2). Interacts with and is activated by binding to NCR3LG1 (PubMed:19528259, PubMed:21422170, PubMed:21444796). Interacts with and is activated by binding to BAG6 (PubMed:18055229, PubMed:18852879). Interacts with and is inhibited by binding to LGALS3 (PubMed:25315772)

Gene: NCR3  |  Organism: Homo sapiens  |  Synonyms: Activating natural killer receptor p30; Natural killer cell p30-related protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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