Recombinant Human T-cell-specific surface glycoprotein CD28 (CD28), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 16.5 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human T-cell-specific surface glycoprotein CD28 (CD28), partial (Active)

Recombinant Human T-cell-specific surface glycoprotein CD28 (CD28), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >90%.

SKU: BCACP-000612 Categories: ,
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Product SKUBCACP-000612
Product DescriptionRecombinant Human T-cell-specific surface glycoprotein CD28 (CD28) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-152aa. [Accession | P10747].
Uniprot No.P10747
Gene NamesCD28
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-152aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight16.5 kDa
ActivityMeasured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The specific activity is >2000 pmol/min/µg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesT-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

Function

Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.

Biological Context

Subcellular Location: Cell surface
Tissue Specificity: Expressed in T-cells and plasma cells, but not in less mature B-cells
Disease Association: Immunodeficiency 123 with HPV-related verrucosis (IMD123) : An autosomal recessive immunologic disorder characterized by susceptibility to human papilloma virus (HPV) infections and the development of HPV-related common verrucosis in the first decade of life. In some patients with HPV2 infection, warts may progress to severe generalized hyperkeratotic cutaneous papillomatosis with cutaneous horns ('tree-man' phenotype). In patients with HPV4 infection, warts remains stable and may even regress with age. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human T-cell-specific surface glycoprotein CD28 (CD28), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-152aa, with C-terminal 10xHis-tagged tag, molecular weight 16.5 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037)

Subcellular Location

Cell surface

Disease Association

Immunodeficiency 123 with HPV-related verrucosis (IMD123) : An autosomal recessive immunologic disorder characterized by susceptibility to human papilloma virus (HPV) infections and the development of HPV-related common verrucosis in the first decade of life. In some patients with HPV2 infection, warts may progress to severe generalized hyperkeratotic cutaneous papillomatosis with cutaneous horns ('tree-man' phenotype). In patients with HPV4 infection, warts remains stable and may even regress with age. [The disease may be caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells

Subunit

Interacts with CD40LG

Gene: CD28  |  Organism: Homo sapiens  |  Synonyms: TP44
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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