Recombinant Human Complement C5 (C5) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 188.0 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Complement C5 (C5) (Active)

Recombinant Human Complement C5 (C5) (Active) — Mediator of local inflammatory process released following cleavage by C5 convertase. Purity >90%.

SKU: BCACP-000614 Category:
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Product SKUBCACP-000614
Product DescriptionRecombinant Human Complement C5 (C5) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-1676aa. [Accession | P01031].
Uniprot No.P01031
Gene NamesC5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-1676aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight188.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody . The EC50 is 38.88-61.07 ng/mL.;Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET . The EC50 is 2.471-2.843 ng/mL.;HGF Recombinant Monoclonal Antibody captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200).;Recombinant Human MET captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesComplement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 [Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5b; Complement C5 alpha' chain]

Function

Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Biological Context

Subcellular Location: Secreted
Disease Association: Complement component 5 deficiency (C5D) : A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. [The disease is caused by variants affecting the gene represented in this entry] | [An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele]

Product Specifications

Recombinant Human Complement C5 (C5) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-1676aa, with C-terminal 10xHis-tagged tag, molecular weight 188.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed:8182049, PubMed:9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:36806352, PubMed:37852260, PubMed:37169960, PubMed:8182049, PubMed:9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed:342601, PubMed:37852260, PubMed:37169960, PubMed:5765461, PubMed:8182049, PubMed:9553099)

Subcellular Location

Secreted

Disease Association

Complement component 5 deficiency (C5D) : A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. [The disease is caused by variants affecting the gene represented in this entry] | [An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele]

Subunit

(Microbial infection) Interacts with Staphylococcus aureus protein SSL5

Gene: C5  |  Organism: Homo sapiens  |  Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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