Recombinant Bovine Hepatocyte growth factor (HGF) (Active), Bos taurus (Bovine), Mammalian cell, C-terminal 10xHis-tagged, 81.2 kDa, purity >95% as determined by SDS-PAGE.
,

Recombinant Bovine Hepatocyte growth factor (HGF) (Active)

Recombinant Bovine Hepatocyte growth factor (HGF) (Active) — Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Purity >95%.

SKU: BCACP-000617 Categories: ,
Online Inquiry →
Product SKUBCACP-000617
Product DescriptionRecombinant Bovine Hepatocyte growth factor (HGF) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 32-730aa. [Accession | Q76BS1].
Uniprot No.Q76BS1
Gene NamesHGF
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region32-730aa
SpeciesBos taurus (Bovine)
Tag InfoC-terminal 10xHis-tagged
Molecular weight81.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human ARG1 at 2 μg/mL can bind Anti-ARG1 recombinant antibody . The EC50 is 2.291-3.018 ng/mL.;ARG1 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human ARG1 with an affinity constant of 0.918 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesHepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

Product Specifications

Recombinant Bovine Hepatocyte growth factor (HGF) (Active) is a recombinant protein from Bos taurus (Bovine), expressed in Mammalian cell, covering amino acids 32-730aa, with C-terminal 10xHis-tagged tag, molecular weight 81.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (By similarity). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity)

Subunit

Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts with SRPX2; the interaction increases HGF mitogenic activity (By similarity)

Gene: HGF  |  Organism: Bos taurus  |  Synonyms: Hepatopoietin-A; Scatter factor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top