Recombinant Mouse Fractalkine protein (Cx3cl1), partial (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 8.7 kDa, purity >>97% as determined by SDS-PAGE.

Recombinant Mouse Fractalkine protein (Cx3cl1), partial (Active)

Recombinant Mouse Fractalkine protein (Cx3cl1), partial (Active) — recombinant protein from Mus musculus (Mouse), expressed in E.Coli. Purity >97%.

SKU: BCACP-000796 Category:
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Product SKUBCACP-000796
Product DescriptionRecombinant Mouse Fractalkine protein (Cx3cl1) protein is expressed from E.Coli with Tag-Free. It contains 25-100aa. [Accession | O35188].
Uniprot No.O35188
Gene NamesCx3cl1
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region25-100aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight8.7 kDa
ActivityFully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCx3cl1; Cx3c; Fkn; Scyd1; Fractalkine; FK; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1

Function

The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis. Highly expressed in lesional smooth muscle cells, but not macrophages. Low levels of ABCD-3 mRNA were also found in anti-CD40-stimulated splenic B-cells, but not in resting B-cells. Also expressed in dendritic cells

Product Specifications

Recombinant Mouse Fractalkine protein (Cx3cl1), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 25-100aa, with Tag-Free tag, molecular weight 8.7 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

The membrane-bound form promotes adhesion of those leukocytes to endothelial cells

Subcellular Location

Secreted

Tissue Specificity

Highest levels in brain (PubMed:10508268, PubMed:9177350, PubMed:9479488, PubMed:9845323). Lower levels in kidney, heart and lung (PubMed:10508268, PubMed:9177350, PubMed:9479488). Also found in skeletal muscle and testis (PubMed:9177350, PubMed:9479488). Highly expressed in lesional smooth muscle cells, but not macrophages (PubMed:12569158). Low levels of ABCD-3 mRNA were also found in anti-CD40-stimulated splenic B-cells, but not in resting B-cells. Also expressed in dendritic cells (PubMed:10508268)

Subunit

Monomer (By similarity). Forms a ternary complex with CX3CR1 and ITGAV:ITGB3 or ITGA4:ITGB1 (By similarity)

Gene: Cx3cl1  |  Organism: Mus musculus  |  Synonyms: ABCD-3; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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