Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 37.5 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active)

Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active) — Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Purity >95%.

SKU: BCACP-001099 Categories: ,
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Product SKUBCACP-001099
Product DescriptionRecombinant Human Malate dehydrogenase, cytoplasmic (MDH1) protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 2-334aa. [Accession | P40925].
Uniprot No.P40925
Gene NamesMDH1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-334aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight37.5 kDa
ActivityThe ED50 as determined by its ability to bind Human EphA2 in functional ELISA is less than 10 ug/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Namescytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase; Malate dehydrogenase cytoplasmic; MDH s; mdh1; MDHA; MDHC_HUMAN; MDHs; MGC:1375; MOR2; Soluble malate dehydrogenase

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.

Product Specifications

Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active) — Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Purity >95%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:2449162, PubMed:3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed:34012073)

Subcellular Location

Cytoplasm, cytosol

Disease Association

Developmental and epileptic encephalopathy 88 (DEE88) : A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homodimer

Gene: MDH1  |  Organism: Homo sapiens  |  Synonyms: Aromatic alpha-keto acid reductase; Cytosolic malate dehydrogenase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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