Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (TNF), partial (Active) (GMP), Homo sapiens (Human), E.Coli, Tag-Free, 17.5 kDa, purity >> 98 % by SDS-PAGE and HPLC analyses.
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Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (TNF), partial (Active) (GMP)

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (TNF), partial (Active) (GMP) — recombinant protein from Homo sapiens (Human), expressed in E.Coli. Purity > 98 % by SDS-PAGE and HPLC analyses..

SKU: BCACP-001256 Categories: ,
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Product SKUBCACP-001256
Product DescriptionRecombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (TNF), (GMP) protein is expressed from E.Coli with Tag-Free. It contains 77-233aa. [Accession | P01375].
Uniprot No.P01375
Gene NamesTNF
Purity> 98 % by SDS-PAGE and HPLC analyses.
Expression SystemE.Coli
Expression Region77-233aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight17.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human FMC63 at 2μg/ml can bind Human CD19-Fc, the ED50 of Recombinant Human CD19-Fc is 55.28 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesAPC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Biological Context

Subcellular Location: Secreted
Disease Association: Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (TNF), partial (Active) (GMP) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 77-233aa, with Tag-Free tag, molecular weight 17.5 kDa, purity > 98 % by SDS-PAGE and HPLC analyses.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells

Subcellular Location

Secreted

Disease Association

Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Subunit

Homotrimer. Interacts with SPPL2B

Gene: TNF  |  Organism: Homo sapiens  |  Synonyms: Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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