Recombinant Mouse Integral membrane protein 2A (Itm2a) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 31.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Integral membrane protein 2A (Itm2a) Protein

Recombinant Mouse Integral membrane protein 2A (Itm2a) Protein — recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system. Purity >85%.

SKU: BCRECP-00086TM Category:
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Product Specifications

Uniprot No.Q61500
Gene NamesItm2a
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-263aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight31.2kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Biological Context

Subcellular Location: Membrane (Single-pass type II membrane protein)
Tissue Specificity: Expressed in mandibular condyles, in bone and in hair follicles. Strong expression in osteogenic tissues, such as neonatal calvaria, paws, tail and skin

Product Specifications

Recombinant Mouse Integral membrane protein 2A (Itm2a) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-263aa, with N-terminal 10xHis-tagged tag, molecular weight 31.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Subcellular Location

Membrane (Single-pass type II membrane protein)

Tissue Specificity

Expressed in mandibular condyles, in bone and in hair follicles. Strong expression in osteogenic tissues, such as neonatal calvaria, paws, tail and skin

Gene: Itm2a  |  Organism: Mus musculus  |  Synonyms: Protein E25

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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