Recombinant Escherichia coli Phospholipase A1 (pldA) Protein, Escherichia coli (strain K12), in vitro E.coli expression system, N-terminal 10xHis-tagged, 32.3kDa, purity >95% as determined by SDS-PAGE.

Recombinant Escherichia coli Phospholipase A1 (pldA) Protein

Recombinant Escherichia coli Phospholipase A1 (pldA) Protein — Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3. Purity >95%.

SKU: BCRECP-00448TM Category:
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Product Specifications

Uniprot No.P0A921
Gene NamespldA
PurityGreater than 95% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region21-289aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged
Molecular weight32.3kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.

Biological Context

Subcellular Location: Cell outer membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Phospholipase A1 (pldA) Protein is a recombinant protein from Escherichia coli (strain K12), expressed in in vitro E.coli expression system, covering amino acids 21-289aa, with N-terminal 10xHis-tagged tag, molecular weight 32.3kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins

Subcellular Location

Cell outer membrane (Multi-pass membrane protein)

Subunit

Homodimer; dimerization is reversible, and the dimeric form is the active one

Gene: pldA  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Detergent-resistant phospholipase A; Outer membrane phospholipase A; Phosphatidylcholine 1-acylhydrolase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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