Recombinant Bovine Gap junction alpha-1 protein(GJA1) Protein, Bos taurus (Bovine), in vitro E.coli expression system, N-terminal 10xHis-tagged, 44.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bovine Gap junction alpha-1 protein(GJA1) Protein

Recombinant Bovine Gap junction alpha-1 protein(GJA1) Protein — Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small… Purity >85%.

SKU: BCRECP-00013TM Category:
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Product Specifications

Uniprot No.P18246
Gene NamesGJA1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region2-383aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged
Molecular weight44.6kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell junction, gap junction; Endoplasmic reticulum; Cell junction

Product Specifications

Recombinant Bovine Gap junction alpha-1 protein(GJA1) Protein is a recombinant protein from Bos taurus (Bovine), expressed in in vitro E.coli expression system, covering amino acids 2-383aa, with N-terminal 10xHis-tagged tag, molecular weight 44.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (By similarity). Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in the conductive system of ventricular myocardium and heart morphogenesis. May play a role in cell growth inhibition through the regulation of NOV expression and localization (By similarity). Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell junction, gap junction; Endoplasmic reticulum; Cell junction

Subunit

A connexon/hemichannel is composed of a hexamer of connexins. Forms functional homotypic gap junctions consisting of identical hemichannels both made of the same connexin type as well as heterotypic gap junctions consisting of two different hemichannels, each containing a different type of connexin. Assembles with GJC1 to form heterotypic gap junction channels (By similarity). Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with SGSM3 and CNST (By similarity). Interacts with RIC1/CIP150. Interacts with CSNK1D. Interacts with NOV (By similarity). Interacts with TMEM65 (By similarity). Interacts with ANK3/ANKG and PKP2 (By similarity)

Gene: GJA1  |  Organism: Bos taurus  |  Synonyms: Connexin-43; Vascular smooth muscle connexin-43

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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