Recombinant African swine fever virus Major structural protein p17 (Ba71V-107) Protein, African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV), in vitro E.coli expression system, N-terminal 10xHis-tagged, 19.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant African swine fever virus Major structural protein p17 (Ba71V-107) Protein

Recombinant African swine fever virus Major structural protein p17 (Ba71V-107) Protein — Together with the penton and the other minor capsid proteins (M1249L, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Purity >85%.

SKU: BCRECP-00723TM Categories: ,
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Product Specifications

Uniprot No.Q89424
Gene NamesBa71V-107
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-117aa
SpeciesAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Tag InfoN-terminal 10xHis-tagged
Molecular weight19.2kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Together with the penton and the other minor capsid proteins (M1249L, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Three copies of p17 encircle each p72 capsomer in the inner capsid shell, anchoring p72 capsomers on the inner membrane. Required for the assembly of the capsid and icosahedral morphogenesis.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein)

Product Specifications

Recombinant African swine fever virus Major structural protein p17 (Ba71V-107) Protein is a recombinant protein from African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV), expressed in in vitro E.coli expression system, covering amino acids 1-117aa, with N-terminal 10xHis-tagged tag, molecular weight 19.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Together with the penton and the other minor capsid proteins (M1249L, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid (PubMed:31624094). Three copies of p17 encircle each p72 capsomer in the inner capsid shell, anchoring p72 capsomers on the inner membrane (PubMed:31624094). Required for the assembly of the capsid and icosahedral morphogenesis (PubMed:20504920). Additionally, inhibits the host cGAS-STING pathway through its interaction with STING1 and subsequent interference of the recruitment of downstream components TBK1 and IKBKE (PubMed:35844609)

Subcellular Location

Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein)

Subunit

Interacts with the minor capsid protein M1249L and with the hexon capsid protein p72 capsomers; these interactions form a rigid zipper structure that stabilizes the capsomers. Interacts with host STING1 (PubMed:35844609)

Organism: African swine fever virus (strain Badajoz 1971 Vero-adapted)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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