Recombinant Human NADPH oxidase 4 (NOX4) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged
,

Recombinant Human NADPH oxidase 4 (NOX4) Protein

Recombinant Human NADPH oxidase 4 (NOX4) Protein — recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system.

SKU: BCRECP-00732TM Categories: ,
Online Inquiry →

Product Specifications

Uniprot No.Q9NPH5
Gene NamesNOX4
Expression Systemin vitro E.coli expression system
Expression Region1-578
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Lacks superoxide-generating NADPH oxidase activity.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, perinuclear region
Tissue Specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells

Product Specifications

Recombinant Human NADPH oxidase 4 (NOX4) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-578, with N-terminal 10xHis-tagged tag. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Lacks superoxide-generating NADPH oxidase activity

Subcellular Location

Cytoplasm; Cytoplasm, perinuclear region

Tissue Specificity

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells

Subunit

Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with PPP1R15A (PubMed:26742780). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (PubMed:39909992)

Gene: NOX4  |  Organism: Homo sapiens  |  Synonyms: Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top