Recombinant Mouse 5'-3' exonuclease PLD3 (Pld3) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 58.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse 5′-3′ exonuclease PLD3 (Pld3) Protein

Recombinant Mouse 5′-3′ exonuclease PLD3 (Pld3) Protein — Can alternatively catalyze the transfer (transphosphatidylation) of a glycerol group between (S,R)-lysophosphatidylglycerol (LPG) and monoacylglycerol (MAG). Purity >85%.

SKU: BCRECP-00739TM Category:
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Product Specifications

Uniprot No.O35405
Gene NamesPld3
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-488aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight58.2kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Can alternatively catalyze the transfer (transphosphatidylation) of a glycerol group between (S,R)-lysophosphatidylglycerol (LPG) and monoacylglycerol (MAG). The reaction is characterized by an R-to-S stereo-inversion which is essential for the resistance of the produced (S,S)-bis(monoacylglycero)phosphates (BMPs) to lysosomal degradation. The resulting BMPs, are phospholipids required for the formation of lysosomal intralumenal vesicles (ILVs) where lipid degradation can occur.

Biological Context

Subcellular Location: Lysosome lumen; Early endosome membrane (Single-pass type II membrane protein); Late endosome membrane (Single-pass type II membrane protein); Endosome membrane (Single-pass type II membrane protein); Endoplasmic reticulum membrane (Single-pass type II membrane protein); Golgi apparatus membrane (Single-pass type II membrane protein)
Tissue Specificity: Expressed at higher level in brain than in non-nervous tissue. Expressed in mature neurons of the forebrain and appears to be turned on at late stages of neurogenesis. Expressed during late neuronal development in the forebrain. Expressed in the pyramidal neurons of the cortex and hippocampus. Low expression in the cerebellum

Product Specifications

Recombinant Mouse 5'-3' exonuclease PLD3 (Pld3) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-488aa, with N-terminal 10xHis-tagged tag, molecular weight 58.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Can alternatively catalyze the transfer (transphosphatidylation) of a glycerol group between (S,R)-lysophosphatidylglycerol (LPG) and monoacylglycerol (MAG). The reaction is characterized by an R-to-S stereo-inversion which is essential for the resistance of the produced (S,S)-bis(monoacylglycero)phosphates (BMPs) to lysosomal degradation. The resulting BMPs, are phospholipids required for the formation of lysosomal intralumenal vesicles (ILVs) where lipid degradation can occur

Subcellular Location

Lysosome lumen; Early endosome membrane (Single-pass type II membrane protein); Late endosome membrane (Single-pass type II membrane protein); Endosome membrane (Single-pass type II membrane protein); Endoplasmic reticulum membrane (Single-pass type II membrane protein); Golgi apparatus membrane (Single-pass type II membrane protein)

Tissue Specificity

Expressed at higher level in brain than in non-nervous tissue. Expressed in mature neurons of the forebrain and appears to be turned on at late stages of neurogenesis. Expressed during late neuronal development in the forebrain (PubMed:9813063). Expressed in the pyramidal neurons of the cortex and hippocampus (PubMed:28128235). Low expression in the cerebellum (PubMed:30312375)

Subunit

Homodimer (PubMed:38537643). Interacts with APP

Gene: Pld3  |  Organism: Mus musculus  |  Synonyms: (S,S)-bis(monoacylglycero)phosphate synthase PLD3; Phospholipase D3; Schwannoma-associated protein 9
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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