Recombinant Human CAS1 domain-containing protein 1 (CASD1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 47.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human CAS1 domain-containing protein 1 (CASD1), partial

Recombinant Human CAS1 domain-containing protein 1 (CASD1), partial — Key enzyme in the biosynthesis of O-acetylated (O-Ac) sialoglycans such as gangliosides O-AcGD3 and O-AcGD2, which affect various processes such as cell-cell interactions, host-pathogen recognition. Purity >90%.

SKU: BCRECP-000235 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-000235
Product DescriptionRecombinant Human CAS1 domain-containing protein 1 (CASD1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 39-313aa. [Accession | Q96PB1].
Uniprot No.Q96PB1
Gene NamesCASD1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region39-313aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight47.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Key enzyme in the biosynthesis of O-acetylated (O-Ac) sialoglycans such as gangliosides O-AcGD3 and O-AcGD2, which affect various processes such as cell-cell interactions, host-pathogen recognition. Catalyzes the transfer of an acetyl group from a donor, the acetyl-coenzyme-A molecule (acetyl-CoA), to the C7/8/9 OH-position of a sialic acid residue. The primary site of O-acetyl group transfer on sialic acid seems to depend on cell type and can be C7, from which the O-acetyl group could subsequently migrate to the C8 and then to the C9 position, or at C9 with possibility of migrating to the C8 and then to the C7 position.

Biological Context

Subcellular Location: Golgi apparatus membrane (Multi-pass membrane protein)
Tissue Specificity: Highly expressed in peripheral B lymphocytes

Product Specifications

Recombinant Human CAS1 domain-containing protein 1 (CASD1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 39-313aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 47.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Key enzyme in the biosynthesis of O-acetylated (O-Ac) sialoglycans such as gangliosides O-AcGD3 and O-AcGD2, which affect various processes such as cell-cell interactions, host-pathogen recognition (PubMed:20947662, PubMed:26169044, PubMed:34208013). Catalyzes the transfer of an acetyl group from a donor, the acetyl-coenzyme-A molecule (acetyl-CoA), to the C7/8/9 OH-position of a sialic acid residue (PubMed:20947662, PubMed:26169044). The primary site of O-acetyl group transfer on sialic acid seems to depend on cell type and can be C7, from which the O-acetyl group could subsequently migrate to the C8 and then to the C9 position, or at C9 with possibility of migrating to the C8 and then to the C7 position (PubMed:20947662, PubMed:26169044). Together with ST8SIA1 (GD3 synthase) it increases the levels of ganglioside Ac-O-7-GD3 (PubMed:20947662). Can transfer the acetyl group from acetyl-CoA to free sialate (N-acetylneuraminate, Neu5Ac) in vitro, but has preferred substrate specificity for CMP-activated sialate (CMP-Neu5Ac), resulting in the formation of 9-O-acetylated CMP-Neu5Ac (CMP-Neu5,9Ac2) (PubMed:26169044). CMP-Neu5,9Ac2 may be used by sialyltransferases as a sialate donor for glycoconjugate acceptors such as ganglioside GD3 (PubMed:26169044). O-acetylation at position C9 of ganglioside GD3 can counteract the pro-apoptotic effects of the ganglioside GD3 in tumor cells (PubMed:12486096)

Subcellular Location

Golgi apparatus membrane (Multi-pass membrane protein)

Tissue Specificity

Highly expressed in peripheral B lymphocytes

Gene: CASD1  |  Organism: Homo sapiens  |  Synonyms: CAS1 domain-containing protein 1; CAS1 protein; Sialate O-acetyltransferase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top