Recombinant Human DnaJ homolog subfamily C member 5 (DNAJC5), Biotinylated, Homo sapiens (Human), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 69.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human DnaJ homolog subfamily C member 5 (DNAJC5), Biotinylated

Recombinant Human DnaJ homolog subfamily C member 5 (DNAJC5), Biotinylated — Acts as a general chaperone in regulated exocytosis. Purity >85%.

SKU: BCRECP-000522 Categories: ,
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Product Specifications

Product SkuBCRECP-000522
Product DescriptionRecombinant Human DnaJ homolog subfamily C member 5 (DNAJC5) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 1-198aa. [Accession | Q9H3Z4].
Uniprot No.Q9H3Z4
Gene NamesDNAJC5
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-198aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight69.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Acts as a general chaperone in regulated exocytosis. Acts as a co-chaperone for the SNARE protein SNAP-25. Involved in the calcium-mediated control of a late stage of exocytosis.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Membrane (Lipid-anchor); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Melanosome; Cell membrane
Tissue Specificity: Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart
Disease Association: Ceroid lipofuscinosis, neuronal, 4B (Kufs type), autosomal dominant (CLN4B) : An adult-onset neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. CLN4B has no visual involvement and is characterized by seizures and other neurologic symptoms. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human DnaJ homolog subfamily C member 5 (DNAJC5), Biotinylated is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-198aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 69.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity)

Subcellular Location

Cytoplasm, cytosol; Membrane (Lipid-anchor); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Melanosome; Cell membrane

Disease Association

Ceroid lipofuscinosis, neuronal, 4B (Kufs type), autosomal dominant (CLN4B) : An adult-onset neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. CLN4B has no visual involvement and is characterized by seizures and other neurologic symptoms. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart

Subunit

Oligomers (PubMed:27452402). Homodimer (By similarity). Interacts with the chaperone complex consisting of HSC70 and SGTA (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (PubMed:28882895). Interacts with SYT1, SYT5 and SYT7, and with SYT9, forming a complex with SNAP25 (By similarity)

Gene: DNAJC5  |  Organism: Homo sapiens  |  Synonyms: Ceroid-lipofuscinosis neuronal protein 4; Cysteine string protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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