Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), Homo sapiens (Human), Yeast, C-terminal GST-tagged, 65.8kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN)

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) — Hydroxylates HIF-1 alpha at ‘Asn-803’ in the C-terminal transactivation domain (CAD). Purity >95%.

SKU: BCRECP-000819 Category:
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Product Specifications

Product SkuBCRECP-000819
Product DescriptionRecombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) Protein is expressed from Yeast with C-terminal GST-tagged. It contains 2-349aa. [Accession | Q9NWT6].
Uniprot No.Q9NWT6
Gene NamesHIF1AN
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-349aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal GST-tagged
Molecular weight65.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Biological Context

Subcellular Location: Nucleus; Cytoplasm; Cytoplasm, perinuclear region

Product Specifications

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 2-349aa, with C-terminal GST-tagged tag, molecular weight 65.8kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation

Subcellular Location

Nucleus; Cytoplasm; Cytoplasm, perinuclear region

Subunit

Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4 (By similarity). Interacts with UBE3A. Interacts with ANKS3 (By similarity). Interacts with NECAB3; the interaction is indirect and seems to be mediated by APBA3

Gene: HIF1AN  |  Organism: Homo sapiens  |  Synonyms: Factor inhibiting HIF-1; Hypoxia-inducible factor asparagine hydroxylase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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