Recombinant Human Keratin,type II cytoskeletal 8 (KRT8), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 66.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Keratin,type II cytoskeletal 8 (KRT8), partial

Recombinant Human Keratin,type II cytoskeletal 8 (KRT8), partial — Structural component of intermediate filaments in simple epithelial cells. Purity >90%.

SKU: BCRECP-001044 Category:
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Product Specifications

Product SkuBCRECP-001044
Product DescriptionRecombinant Human Keratin,type II cytoskeletal 8 (KRT8) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-483aa. [Accession | P05787].
Uniprot No.P05787
Gene NamesKRT8
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-483aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight66.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Structural component of intermediate filaments in simple epithelial cells. Together with its obligate type I partner KRT18, contributes to the formation of the keratin intermediate filament network that provides mechanical stability and resilience to epithelial cells. Also involved in maintaining epithelial integrity and protecting cells from non-mechanical stress, such as apoptosis.

Biological Context

Subcellular Location: Cytoplasm; Nucleus, nucleoplasm; Nucleus matrix; Cytoplasm, cytoskeleton
Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity

Product Specifications

Recombinant Human Keratin,type II cytoskeletal 8 (KRT8), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-483aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 66.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Structural component of intermediate filaments in simple epithelial cells. Together with its obligate type I partner KRT18, contributes to the formation of the keratin intermediate filament network that provides mechanical stability and resilience to epithelial cells (PubMed:26823019). Also involved in maintaining epithelial integrity and protecting cells from non-mechanical stress, such as apoptosis (By similarity). Also required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed:26823019). In striated muscle, together with KRT19, may contribute to linking the contractile apparatus to dystrophin at the costameres (PubMed:16000376)

Subcellular Location

Cytoplasm; Nucleus, nucleoplasm; Nucleus matrix; Cytoplasm, cytoskeleton

Tissue Specificity

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity

Subunit

(Microbial infection) Interacts with hepatitis C virus/HCV core protein

Gene: KRT8  |  Organism: Homo sapiens  |  Synonyms: Cytokeratin-8; Keratin-8; Type-II keratin Kb8
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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