Recombinant Human Spindlin-4 (SPIN4), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 28.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Spindlin-4 (SPIN4), partial

Recombinant Human Spindlin-4 (SPIN4), partial — Binds to acetylated and methylated histones, including H3K4me3 and H4K20me3, probably acting as a histone reader that recognizes chromatin marks and mediates downstream cellular effects. Purity >85%.

SKU: BCRECP-001689 Category:
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Product Specifications

Product SkuBCRECP-001689
Product DescriptionRecombinant Human Spindlin-4 (SPIN4) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 36-249aa. [Accession | Q56A73].
Uniprot No.Q56A73
Gene NamesSPIN4
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region36-249aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Binds to acetylated and methylated histones, including H3K4me3 and H4K20me3, probably acting as a histone reader that recognizes chromatin marks and mediates downstream cellular effects. Promotes canonical WNT signaling, and is involved in the down-regulation of cell proliferation.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Disease Association: Lui-Jee-Baron syndrome (LJBS) : An X-linked disorder characterized by prenatal onset, generalized overgrowth, extreme tall stature, enlarged liver and spleen, macrocephaly, dysmorphic features, and normal development. Hemizygous males are more severely affected than heterozygous females. [The disease may be caused by variants affecting the gene represented in this entry. A variant causing frameshift and truncation of the SPIN4 protein has been found in one family with Lui-Jee-Baron syndrome. In mice, SPIN4 truncating mutations result in features recapitulating the human disease, including generalized overgrowth and increased longitudinal bone growth. Growth plate analysis of mutant mice reveales increased cell proliferation in the proliferative zone and an increased number of progenitor chondrocytes in the resting zone]

Product Specifications

Recombinant Human Spindlin-4 (SPIN4), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 36-249aa, with N-terminal 6xHis-tagged tag, molecular weight 28.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Binds to acetylated and methylated histones, including H3K4me3 and H4K20me3, probably acting as a histone reader that recognizes chromatin marks and mediates downstream cellular effects (PubMed:29061846, PubMed:36927955). Promotes canonical WNT signaling, and is involved in the down-regulation of cell proliferation (PubMed:36927955)

Subcellular Location

Cytoplasm; Nucleus

Disease Association

Lui-Jee-Baron syndrome (LJBS) : An X-linked disorder characterized by prenatal onset, generalized overgrowth, extreme tall stature, enlarged liver and spleen, macrocephaly, dysmorphic features, and normal development. Hemizygous males are more severely affected than heterozygous females. [The disease may be caused by variants affecting the gene represented in this entry. A variant causing frameshift and truncation of the SPIN4 protein has been found in one family with Lui-Jee-Baron syndrome. In mice, SPIN4 truncating mutations result in features recapitulating the human disease, including generalized overgrowth and increased longitudinal bone growth. Growth plate analysis of mutant mice reveales increased cell proliferation in the proliferative zone and an increased number of progenitor chondrocytes in the resting zone]

Subunit

Interacts with C11orf84/SPINDOC (PubMed:29061846). Associates with chromatin (PubMed:36927955)

Gene: SPIN4  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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