Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 56.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), partial

Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), partial — Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Purity >90%.

SKU: BCRECP-001943 Category:
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Product Specifications

Product SkuBCRECP-001943
Product DescriptionRecombinant Human Zona pellucida sperm-binding protein 3 (ZP3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 23-387aa. [Accession | P21754].
Uniprot No.P21754
Gene NamesZP3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region23-387aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight56.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed in oocytes (at protein level)
Disease Association: Oocyte/zygote/embryo maturation arrest 3 (OZEMA3) : An autosomal dominant infertility disorder characterized by abnormal oocytes that lack the zona pellucida, and oocytes degeneration. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 23-387aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 56.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Disease Association

Oocyte/zygote/embryo maturation arrest 3 (OZEMA3) : An autosomal dominant infertility disorder characterized by abnormal oocytes that lack the zona pellucida, and oocytes degeneration. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in oocytes (at protein level)

Subunit

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP1 and ZP2 (PubMed:28886344)

Gene: ZP3  |  Organism: Homo sapiens  |  Synonyms: Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3; Zona pellucida protein C
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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