Recombinant Apis mellifera Major royal jelly protein 1 (MRJP1) — Lacks antibacterial and antifungal activity. Purity >85%.
| Product Sku | BCRECP-003412 |
| Product Description | Recombinant Apis mellifera Major royal jelly protein 1 (MRJP1) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged. It contains 20-432aa. [Accession | O18330]. |
| Uniprot No. | O18330 |
| Gene Names | MRJP1 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Expression System | Baculovirus |
| Expression Region | 20-432aa |
| Species | Apis mellifera (Honeybee) |
| Tag Info | N-terminal 10xHis-tagged |
| Molecular weight | 49.4kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Lacks antibacterial and antifungal activity. Lacks cytolytic activity and does not induce rat peritoneal mast cell degranulation.
Recombinant Apis mellifera Major royal jelly protein 1 (MRJP1) — Lacks antibacterial and antifungal activity. Purity >85%. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Lacks antibacterial and antifungal activity. Lacks cytolytic activity and does not induce rat peritoneal mast cell degranulation
Secreted; Cytoplasm; Cell projection, rhabdomere; Cytoplasm, cytoskeleton
Found in and secreted from the hypopharyngeal glands of the worker honey bee (at protein level); expression peaks at 12 days post eclosion (PubMed:15203237, PubMed:15607658, PubMed:31410279, PubMed:9395329). Expressed in the brains of worker bees (at protein level); found in antennal lobe, optical lobe and a subpopulation of Kenyon cells in the mushroom body (PubMed:17065613, PubMed:19203288, PubMed:36414079, PubMed:9722965). Found in the ommatidia of worker bees (at protein level) (PubMed:19203288). Expressed in the spermatheca of adult queen bees (at protein level); expression levels are higher in mated queens than in virgin queens (PubMed:34442256). Expressed in queen bee ovaries and male drone testes (PubMed:17065613)
Is present in royal jelly in different forms: monomer (55 kDa), oligomeric subunit (ca. 287-420 kDa), and water-insoluble aggregates in sediment after interaction with fatty acids (PubMed:28252287, PubMed:30135511, Ref.11). Component of the apisin heterooligomer complex consisting of 4 copies of MRJP1 and 4 copies of apisimin, associated with 8 molecules of 24-methylenecholesterol; apisimin forms a bridge connecting two MRJP1 dimers (PubMed:20017154, PubMed:27721892, PubMed:28252287, PubMed:29551410, PubMed:30135511, PubMed:33293513). At low pH multiple apisin octamers stack to form filaments that increase the viscosity of royal jelly; these filaments may be stabilized by bound fatty acid chains (PubMed:29551410, PubMed:30135511, PubMed:33293513). The mandibular gland, where royal jelly is produced, has low pH conditions favouring filament formation, while the higher pH of the insect midgut favors filament disassembly (Probable)
Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.
Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
