Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial, Avian infectious bronchitis virus (strain UK/123/82) (IBV), Mammalian cell, C-terminal 6xHis-tagged, 27kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial

Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial — S2 is a class I viral fusion protein. Purity >90%.

SKU: BCRECP-004325 Categories: , , ,
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Product Specifications

Product SkuBCRECP-004325
Product DescriptionRecombinant Avian infectious bronchitis virus Spike glycoprotein (S) Protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 300-520aa. [Accession | P30206].
Uniprot No.P30206
Gene NamesS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region300-520aa
SpeciesAvian infectious bronchitis virus (strain UK/123/82) (IBV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight27kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Biological Context

Subcellular Location: Virion membrane (Peripheral membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein)

Product Specifications

Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial is a recombinant protein from Avian infectious bronchitis virus (strain UK/123/82) (IBV), expressed in Mammalian cell, covering amino acids 300-520aa, with C-terminal 6xHis-tagged tag, molecular weight 27kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity)

Subcellular Location

Virion membrane (Peripheral membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein)

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity)

Gene: S  |  Organism: Avian infectious bronchitis virus (strain UK/123/82)  |  Synonyms: E2; Peplomer protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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