Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB), Shigella flexneri, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 46.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB)

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) — Required for efficient dissemination. Purity >85%.

SKU: BCRECP-004385 Category:
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Product Specifications

Product SkuBCRECP-004385
Product DescriptionRecombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-363aa. [Accession | P18012].
Uniprot No.P18012
Gene NamessctB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-363aa
SpeciesShigella flexneri
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight46.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Contribute to actin nucleation in vitro, which may be a necessary step in Shigella invasion.

Biological Context

Subcellular Location: Secreted; Host membrane (Single-pass membrane protein)

Product Specifications

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-363aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 46.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Required for efficient dissemination (PubMed:11207551). Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread (PubMed:11207551). Contribute to actin nucleation in vitro, which may be a necessary step in Shigella invasion (PubMed:18656530)

Subcellular Location

Secreted; Host membrane (Single-pass membrane protein)

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10545510, PubMed:17367391). Interacts with IpaB/SctE (PubMed:10545510, PubMed:17367391, PubMed:7954817). Interacts with the molecular chaperone IpgC, which prevents premature association with IpaB/SctE within the cytoplasm of Shigella cells (PubMed:11207551, PubMed:20937829, PubMed:7954817). Does not interact with CDC42 or RAC1 GTPases in vitro (PubMed:18656530)

Gene: sctB  |  Organism: Shigella flexneri  |  Synonyms: 42 kDa antigen; Invasin IpaC; Invasion plasmid antigen C
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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