Recombinant Mouse Transcription factor A, mitochondrial (Tfam), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Transcription factor A, mitochondrial (Tfam)

Recombinant Mouse Transcription factor A, mitochondrial (Tfam) — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >90%.

SKU: BCRECP-004544 Category:
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Product Specifications

Product SkuBCRECP-004544
Product DescriptionRecombinant Mouse Transcription factor A, mitochondrial (Tfam) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 43-243aa. [Accession | P40630].
Uniprot No.P40630
Gene NamesTfam
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region43-243aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

May also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific

Product Specifications

Recombinant Mouse Transcription factor A, mitochondrial (Tfam) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 43-243aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis

Subcellular Location

Nucleus

Tissue Specificity

The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific

Subunit

Monomer; binds DNA as a monomer. Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M. Interacts with CLPX; this enhances DNA-binding

Gene: Tfam  |  Organism: Mus musculus  |  Synonyms: Testis-specific high mobility group protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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