Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2), Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 70.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2)

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) — Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Purity >85%.

SKU: BCRECP-004728 Category:
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Product Specifications

Product SkuBCRECP-004728
Product DescriptionRecombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-556aa. [Accession | P08062].
Uniprot No.P08062
Gene NamesVIRE2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-556aa
SpeciesAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight70.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.

Biological Context

Subcellular Location: Secreted; Host nucleus

Product Specifications

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) is a recombinant protein from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)), expressed in E.coli, covering amino acids 1-556aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 70.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition

Subcellular Location

Secreted; Host nucleus

Subunit

Interacts with host VIP2 that promotes T-DNA integration into the host genome. Forms a complex made of virE2 and host proteins VIP1 and VBF (By similarity). Forms heterodimers with the chaperone protein virE1 that prevent virE2 anarchic homopolymerization. Interacts with A.thaliana VIP1 that mediates its translocation to the host nucleus. Forms a complex made of VirE2, host VIP1 and VIP2 and single-stranded DNA (ssDNA)

Gene: virE2  |  Organism: Agrobacterium fabrum (strain C58 / ATCC 33970)  |  Synonyms: 63.5 kDa virulence protein
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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