Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial, Agrobacterium tumefaciens (strain 15955), E.coli, C-terminal 6xHis-tagged, 31.4kDa, purity >95% as determined by SDS-PAGE.

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial — Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Purity >95%.

SKU: BCRECP-004729 Category:
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Product Specifications

Product SkuBCRECP-004729
Product DescriptionRecombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 316-533aa. [Accession | P0A3W9].
Uniprot No.P0A3W9
Gene NamesvirE2
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region316-533aa
SpeciesAgrobacterium tumefaciens (strain 15955)
Tag InfoC-terminal 6xHis-tagged
Molecular weight31.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.

Biological Context

Subcellular Location: Secreted; Host nucleus

Product Specifications

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial is a recombinant protein from Agrobacterium tumefaciens (strain 15955), expressed in E.coli, covering amino acids 316-533aa, with C-terminal 6xHis-tagged tag, molecular weight 31.4kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition (By similarity)

Subcellular Location

Secreted; Host nucleus

Subunit

Forms heterodimers with the chaperone protein virE1 that prevent virE2 anarchic homopolymerization. Interacts with host VIP1 that mediates its translocation to the host nucleus and host VIP2 that promotes T-DNA integration into the host genome. Forms a complex made of VirE2, host VIP1 and VIP2 and single-stranded DNA (ssDNA). Forms a complex made of virE2 and host proteins VIP1 and VBF (By similarity)

Gene: virE2  |  Organism: Agrobacterium tumefaciens (strain 15955)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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