Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial, Agrobacterium tumefaciens (strain 15955), E.coli, N-terminal 6xHis-tagged, 30.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial — Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Purity >90%.

SKU: BCRECP-004730 Category:
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Product Specifications

Product SkuBCRECP-004730
Product DescriptionRecombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 316-533aa. [Accession | P0A3W9].
Uniprot No.P0A3W9
Gene NamesvirE2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region316-533aa
SpeciesAgrobacterium tumefaciens (strain 15955)
Tag InfoN-terminal 6xHis-tagged
Molecular weight30.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.

Biological Context

Subcellular Location: Secreted; Host nucleus

Product Specifications

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial is a recombinant protein from Agrobacterium tumefaciens (strain 15955), expressed in E.coli, covering amino acids 316-533aa, with N-terminal 6xHis-tagged tag, molecular weight 30.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition (By similarity)

Subcellular Location

Secreted; Host nucleus

Subunit

Forms heterodimers with the chaperone protein virE1 that prevent virE2 anarchic homopolymerization. Interacts with host VIP1 that mediates its translocation to the host nucleus and host VIP2 that promotes T-DNA integration into the host genome. Forms a complex made of VirE2, host VIP1 and VIP2 and single-stranded DNA (ssDNA). Forms a complex made of virE2 and host proteins VIP1 and VBF (By similarity)

Gene: virE2  |  Organism: Agrobacterium tumefaciens (strain 15955)

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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