Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1), Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 44.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)

Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) — Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Purity >85%.

SKU: BCRECP-002817 Categories: ,
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Product Specifications

Product SkuBCRECP-002817
Product DescriptionRecombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-338aa. [Accession | P25858].
Uniprot No.P25858
Gene NamesGAPC1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-338aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight44.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Expressed in leaves, stems and siliques and at lower levels in roots and flowers
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Product Specifications

Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1) is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 1-338aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 44.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility (PubMed:18820081). Involved in response to oxidative stress by mediating plant responses to abscisic acid (ABA) and water deficits through the activation of PLDDELTA and production of phosphatidic acid (PA), a multifunctional stress signaling lipid in plants (PubMed:22589465). Associates with FBA6 to the outer mitochondrial membrane, in a redox-dependent manner, leading to binding and bundling of actin. Actin binding and bundling occurs under oxidizing conditions and is reversible under reducing conditions. May be part of a redox-dependent retrograde signal transduction network for adaptation upon oxidative stress (PubMed:23316205). Binds DNA in vitro. Together with DNA polymerase II subunit B3-1 (DPB3-1) and GAPC2, enhances heat tolerance and promotes the expression of heat-inducible genes (PubMed:32651385)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Expressed in leaves, stems and siliques and at lower levels in roots and flowers

Subunit

Homotetramer (By similarity). Interacts with PLDDELTA (PubMed:22589465). Interacts with FBA6 and VDAC3 (PubMed:23316205). Binds to DPB3-1/NF-YC10 in response to heat-stress; this interaction promotes DPB3-1/NF-YC10 DNA-binding ability to its target promoter (PubMed:32651385)

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Gene: GAPC1  |  Organism: Arabidopsis thaliana  |  Synonyms: NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 1
Key Publications

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