Recombinant Escherichia coli 30S ribosomal protein S4 (rpsD), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 27.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli 30S ribosomal protein S4 (rpsD)

Recombinant Escherichia coli 30S ribosomal protein S4 (rpsD) — Part of the processive rRNA transcription and antitermination complex (rrnTAC). Purity >90%.

SKU: BCRECP-004296 Category:
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Product Specifications

Product SkuBCRECP-004296
Product DescriptionRecombinant Escherichia coli 30S ribosomal protein S4 (rpsD) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-206aa. [Accession | P0A7V8].
Uniprot No.P0A7V8
Gene NamesrpsD
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-206aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight27.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.

Product Specifications

Recombinant Escherichia coli 30S ribosomal protein S4 (rpsD) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 2-206aa, with N-terminal 6xHis-tagged tag, molecular weight 27.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. This subunit may play a particular role in long-distance rRNA annealing needed for pre-rRNA processing

Subunit

Part of the 30S ribosomal subunit (PubMed:10094780, PubMed:1100394, PubMed:12244297, PubMed:12809609, PubMed:16272117, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875, PubMed:4587210, PubMed:7556101). Contacts protein S5. With proteins S3 and S5 encircles the mRNA as it enters the ribosome, which may play a role in mRNA helicase processivity (PubMed:15652481). Some nascent polypeptide chains are able to cross-link to this protein in situ (PubMed:9716382). The rRNA transcription and antitermination complex (rrnTAC) consists of RNAP, NusA, NusB, NusE (rpsJ), NusG, SubB, ribosomal protein S4, DNA and precursor rRNA; S4 is more flexible than other subunits (PubMed:32871103)

Gene: rpsD  |  Organism: Escherichia coli (strain K12)  |  Synonyms: 30S ribosomal protein S4
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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