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Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial

Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial — Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Purity >85%.

SKU: BCRECP-002509 Categories: ,

Product Specifications

Product SkuBCRECP-002509
Product DescriptionRecombinant Human adenovirus C serotype 2 DNA-binding protein (DBP) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 174-529aa. [Accession | P03264].
Uniprot No.P03264
Gene NamesDBP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region174-529aa
SpeciesHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis.

Biological Context

Subcellular Location: Host nucleus

Product Specifications

Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial is a recombinant protein from Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2), expressed in E.coli, covering amino acids 174-529aa, with N-terminal 6xHis-tagged tag, molecular weight 45.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its release from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP

Subcellular Location

Host nucleus

Subunit

Homomultimerizes on viral ssDNA bound to pTP (PubMed:9135160). Forms an initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1 (By similarity). Interacts with host SRCAP (PubMed:11581372)

Gene: DBP  |  Organism: Human adenovirus C serotype 2  |  Synonyms: Early 2A protein; Early E2A DNA-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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