Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial, Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2), E.coli, N-terminal 6xHis-tagged, 45.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial

Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial — Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Purity >85%.

SKU: BCRECP-002509 Categories: ,
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Product Specifications

Product SkuBCRECP-002509
Product DescriptionRecombinant Human adenovirus C serotype 2 DNA-binding protein (DBP) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 174-529aa. [Accession | P03264].
Uniprot No.P03264
Gene NamesDBP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region174-529aa
SpeciesHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis.

Biological Context

Subcellular Location: Host nucleus

Product Specifications

Recombinant Human adenovirus C serotype 2 DNA-binding protein (DBP), partial is a recombinant protein from Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2), expressed in E.coli, covering amino acids 174-529aa, with N-terminal 6xHis-tagged tag, molecular weight 45.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its release from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP

Subcellular Location

Host nucleus

Subunit

Homomultimerizes on viral ssDNA bound to pTP (PubMed:9135160). Forms an initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1 (By similarity). Interacts with host SRCAP (PubMed:11581372)

Gene: DBP  |  Organism: Human adenovirus C serotype 2  |  Synonyms: Early 2A protein; Early E2A DNA-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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