Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 10.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial

Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial — Translocates drugs and phospholipids across the membrane. Purity >90%.

SKU: BCRECP-000003 Category:
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Product Specifications

Product SkuBCRECP-000003
Product DescriptionRecombinant Human ATP-dependent translocase ABCB1 (ABCB1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 236-297aa. [Accession | P08183].
Uniprot No.P08183
Gene NamesABCB1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region236-297aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight10.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTransport

Function

Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Apical cell membrane; Cytoplasm
Tissue Specificity: Expressed in small intestine. Expressed in liver, kidney and brain
Disease Association: Inflammatory bowel disease 13 (IBD13) : A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Encephalopathy, acute transient (ENPAT) : An autosomal recessive neurologic disorder triggered by specific drugs, or acute febrile or afebrile illness. Clinical features include encephalopathy, ataxia, spasticity, pyramidal signs, diplopia, vomiting, and coma. Symptoms fully resolve within few days. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 236-297aa, with N-terminal 6xHis-tagged tag, molecular weight 10.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218, PubMed:35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Apical cell membrane; Cytoplasm

Disease Association

Inflammatory bowel disease 13 (IBD13) : A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Encephalopathy, acute transient (ENPAT) : An autosomal recessive neurologic disorder triggered by specific drugs, or acute febrile or afebrile illness. Clinical features include encephalopathy, ataxia, spasticity, pyramidal signs, diplopia, vomiting, and coma. Symptoms fully resolve within few days. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in small intestine (PubMed:28408210). Expressed in liver, kidney and brain

Subunit

Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1

Gene: ABCB1  |  Organism: Homo sapiens  |  Synonyms: ATP-binding cassette sub-family B member 1; Multidrug resistance protein 1; P-glycoprotein 1; Phospholipid transporter ABCB1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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