Recombinant Human B-lymphocyte antigen CD19 (CD19), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc-tagged, 57.3 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human B-lymphocyte antigen CD19 (CD19), partial (Active)

Recombinant Human B-lymphocyte antigen CD19 (CD19), partial (Active) — Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Purity >95%.

SKU: BCACP-001261 Categories: ,
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Product SKUBCACP-001261
Product DescriptionRecombinant Human B-lymphocyte antigen CD19 (CD19) protein is expressed from Mammalian cell with C-terminal hFc-tagged. It contains 20-291aa. [Accession | P15391].
Uniprot No.P15391
Gene NamesCD19
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region20-291aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc-tagged
Molecular weight57.3 kDa
ActivityLoaded Human IgG1 Fc on Protein-A Biosensor, can bind Human CD16a-His (V176) with an affinity constant of 0.571 uM as determined in BLI assay.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD antigen CD19

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Membrane raft (Single-pass type I membrane protein)
Tissue Specificity: Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level)
Disease Association: Immunodeficiency, common variable, 3 (CVID3) : A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human B-lymphocyte antigen CD19 (CD19), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 20-291aa, with C-terminal hFc-tagged tag, molecular weight 57.3 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Loaded Human IgG1 Fc on Protein-A Biosensor, can bind Human CD16a-His (V176) with an affinity constant of 0.571 uM as determined in BLI assay.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Membrane raft (Single-pass type I membrane protein)

Disease Association

Immunodeficiency, common variable, 3 (CVID3) : A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:16672701, PubMed:2463100)

Subunit

Interacts with CR2/CD21 (PubMed:1702139). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells (PubMed:1383329). Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells (PubMed:16449649). Interacts with VAV (PubMed:10706702). Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409 (PubMed:10706702). Interacts with LYN (PubMed:7687428). Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2) (PubMed:7684160). Interacts with GRB2 (PubMed:29523808)

Gene: CD19  |  Organism: Homo sapiens  |  Synonyms: B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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