Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal 10xHis-tagged, 24.6 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.

Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial (Active)

Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial (Active) — (Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. Purity >95%.

SKU: BCACP-000660 Category:
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Product SKUBCACP-000660
Product DescriptionRecombinant Human C-type lectin domain family 5 member A (CLEC5A) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 28-188aa. [Accession | Q9NY25].
Uniprot No.Q9NY25
Gene NamesCLEC5A
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region28-188aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight24.6 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CLEC7A at 2 μg/mL can bind Anti-CLEC7A recombinant antibody . The EC50 is 1.715-1.991 ng/mL.;CLEC7A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human CLEC7A with an affinity constant of 0.807 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesC-type lectin domain family 5 member A; C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1; MDL-1

Function

(Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP. This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)
Tissue Specificity: Highly expressed in bone marrow with lower levels in synovium, lung and bronchus. Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells

Product Specifications

Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 28-188aa, with N-terminal 10xHis-tagged tag, molecular weight 24.6 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC7A at 2 μg/mL can bind Anti-CLEC7A recombinant antibody . The EC50 is 1.715-1.991 ng/mL.;CLEC7A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human CLEC7A with an affinity constant of 0.807 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

(Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4 (PubMed:18496526, PubMed:21566123). The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP (PubMed:18496526). This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release (PubMed:18496526)

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Tissue Specificity

Highly expressed in bone marrow with lower levels in synovium, lung and bronchus (PubMed:20212065). Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins (PubMed:10449773). Expression is down-regulated when monocytes differentiate into dendritic cells (PubMed:10449773)

Subunit

(Microbial infection) Interacts with dengue virus envelope protein E (PubMed:18496526, PubMed:21566123)

Gene: CLEC5A  |  Organism: Homo sapiens  |  Synonyms: C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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