Recombinant Human CD166 antigen (ALCAM), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal mFc2a-tagged, 85.3 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human CD166 antigen (ALCAM), partial (Active)

Recombinant Human CD166 antigen (ALCAM), partial (Active) — Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Purity >95%.

SKU: BCACP-000658 Categories: ,
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Product SKUBCACP-000658
Product DescriptionRecombinant Human CD166 antigen (ALCAM) protein is expressed from Mammalian cell with C-terminal mFc2a-tagged. It contains 28-526aa. [Accession | Q13740].
Uniprot No.Q13740
Gene NamesALCAM
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region28-526aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal mFc2a-tagged
Molecular weight85.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human IL10RA at 2 μg/mL can bind Human IL10 . The EC50 is 2.897-3.185 ng/mL.The VLPs is negative control.;Human IL10 captured on Protein A Chip can bind Recombinant Human IL10RA with an affinity constant of 85.8 pM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD166 antigen; Activated leukocyte cell adhesion molecule; CD antigen CD166

Function

Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Detected on hematopoietic stem cells derived from umbilical cord blood. Detected on lymph vessel endothelial cells, skin and tonsil. Detected on peripheral blood monocytes. Detected on monocyte-derived dendritic cells (at protein level). Detected at low levels in spleen, placenta, liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines

Product Specifications

Recombinant Human CD166 antigen (ALCAM), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 28-526aa, with C-terminal mFc2a-tagged tag, molecular weight 85.3 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation

Subcellular Location

Secreted

Tissue Specificity

Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813). Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771). Detected on peripheral blood monocytes (PubMed:15048703). Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806). Detected at low levels in spleen, placenta, liver (PubMed:9502422). Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007). Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415). Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422)

Subunit

Homodimer (PubMed:15048703, PubMed:16352806, PubMed:26146185, PubMed:7760007). Interacts (via extracellular domain) with CD6 (via extracellular domain) (PubMed:15048703, PubMed:16914752, PubMed:24945728, PubMed:26146185, PubMed:7760007, PubMed:8823162). Homodimerization and interaction with CD6 involve the same region and cannot occur simultaneously. The affinity for CD6 is much higher than the affinity for self-association (PubMed:15048703). Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3 (PubMed:24945728). Interaction with LGALS1 or LGALS3 inhibits interaction with CD6 (PubMed:24945728)

Gene: ALCAM  |  Organism: Homo sapiens  |  Synonyms: Activated leukocyte cell adhesion molecule
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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