Recombinant Human CD40 ligand (CD40LG) (C194W), partial (Active), Homo sapiens (Human), E.Coli, Tag-Free, 16.3 kDa, purity >>95% as determined by SDS-PAGE.
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Recombinant Human CD40 ligand (CD40LG) (C194W), partial (Active)

Recombinant Human CD40 ligand (CD40LG) (C194W), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in E.Coli. Purity >95%.

SKU: BCACP-000962 Categories: ,
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Product SKUBCACP-000962
Product DescriptionRecombinant Human CD40 ligand (CD40LG) (C194W) protein is expressed from E.Coli with Tag-Free. It contains 113-261aa(C194W). [Accession | P29965].
Uniprot No.P29965
Gene NamesCD40LG
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region113-261aa(C194W)
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight16.3 kDa
ActivityFully biologically active when compared to standard. The biological activity is determined by its ability to block human BAFF induced T2B cell survival using a concentration range of 1.0-3.0 ug/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD 40L; CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand; soluble form; CD40-L; CD40L; CD40L_HUMAN; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell activating molecule; T BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TrAP; Tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Product Specifications

Recombinant Human CD40 ligand (CD40LG) (C194W), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in E.Coli. Purity >95%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling

Subcellular Location

Secreted

Disease Association

Immunodeficiency with hyper-IgM, type 1 (HIGM1) : Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Specifically expressed on activated CD4+ T-lymphocytes

Subunit

Homotrimer (PubMed:11676606, PubMed:8589998, PubMed:8626375). Interacts with isoform 3 of CD28 (PubMed:15067037). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (PubMed:31331973, PubMed:8626375). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (PubMed:31331973)

Gene: CD40LG  |  Organism: Homo sapiens  |  Synonyms: T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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