Recombinant Human CD40 ligand (CD40LG), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal hFc-Flag-tagged, 44.5 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human CD40 ligand (CD40LG), partial (Active)

Recombinant Human CD40 ligand (CD40LG), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >90%.

SKU: BCACP-000083 Categories: ,
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Product SKUBCACP-000083
Product DescriptionRecombinant Human CD40 ligand (CD40LG) protein is expressed from Mammalian cell with N-terminal hFc-Flag-tagged. It contains 113-261aa. [Accession | P29965].
Uniprot No.P29965
Gene NamesCD40LG
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region113-261aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal hFc-Flag-tagged
Molecular weight44.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized RBP4 at 5 μg/ml can bind TTR , the EC50 is 695.0-970.1 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD40L; TNFSF5; TRAP

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes
Disease Association: Immunodeficiency with hyper-IgM, type 1 (HIGM1) : Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human CD40 ligand (CD40LG), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 113-261aa, with N-terminal hFc-Flag-tagged tag, molecular weight 44.5 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized RBP4 at 5 μg/ml can bind TTR , the EC50 is 695.0-970.1 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling

Subcellular Location

Secreted

Disease Association

Immunodeficiency with hyper-IgM, type 1 (HIGM1) : Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Specifically expressed on activated CD4+ T-lymphocytes

Subunit

Homotrimer (PubMed:11676606, PubMed:8589998, PubMed:8626375). Interacts with isoform 3 of CD28 (PubMed:15067037). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (PubMed:31331973, PubMed:8626375). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (PubMed:31331973)

Gene: CD40LG  |  Organism: Homo sapiens  |  Synonyms: T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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