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Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Fluorescent (Active)

Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Fluorescent (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell.

SKU: BCACP-000230 Categories: ,
Product SKUBCACP-000230
Product DescriptionRecombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Fluorescent protein is expressed from Mammalian cell with C-terminal mCherry-tagged. It contains 1-261aa. [Accession | P56856].
Uniprot No.P56856
Gene NamesCLDN18.2
Expression SystemMammalian cell
Expression Region1-261aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal mCherry-tagged
Molecular weight52.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CD70 at 2 μg/mL can bind Anti-CD70 antibody, the EC50 is 2.414-3.196 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(CLDN18.2)

Function

Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.

Biological Context

Subcellular Location: Cell junction, tight junction; Lateral cell membrane
Tissue Specificity: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level)

Product Specifications

Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Fluorescent (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-261aa, with C-terminal mCherry-tagged tag, molecular weight 52.3 kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification

Subcellular Location

Cell junction, tight junction; Lateral cell membrane

Tissue Specificity

Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level)

Subunit

Interacts with CLDN19

Gene: CLDN18  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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