Recombinant Human Complement C5 (C5) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 188.0 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Complement C5 (C5) (Active)

Recombinant Human Complement C5 (C5) (Active) — Mediator of local inflammatory process released following cleavage by C5 convertase. Purity >90%.

SKU: BCACP-000614 Category:
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Product SKUBCACP-000614
Product DescriptionRecombinant Human Complement C5 (C5) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-1676aa. [Accession | P01031].
Uniprot No.P01031
Gene NamesC5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-1676aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight188.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody . The EC50 is 38.88-61.07 ng/mL.;Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET . The EC50 is 2.471-2.843 ng/mL.;HGF Recombinant Monoclonal Antibody captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200).;Recombinant Human MET captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesComplement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 [Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5b; Complement C5 alpha' chain]

Function

Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Biological Context

Subcellular Location: Secreted
Disease Association: Complement component 5 deficiency (C5D) : A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. [The disease is caused by variants affecting the gene represented in this entry] | [An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele]

Product Specifications

Recombinant Human Complement C5 (C5) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-1676aa, with C-terminal 10xHis-tagged tag, molecular weight 188.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody . The EC50 is 38.88-61.07 ng/mL.;Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET . The EC50 is 2.471-2.843 ng/mL.;HGF Recombinant Monoclonal Antibody captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200).;Recombinant Human MET captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed:8182049, PubMed:9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:36806352, PubMed:37852260, PubMed:37169960, PubMed:8182049, PubMed:9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed:342601, PubMed:37852260, PubMed:37169960, PubMed:5765461, PubMed:8182049, PubMed:9553099)

Subcellular Location

Secreted

Disease Association

Complement component 5 deficiency (C5D) : A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. [The disease is caused by variants affecting the gene represented in this entry] | [An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele]

Subunit

(Microbial infection) Interacts with Staphylococcus aureus protein SSL5

Gene: C5  |  Organism: Homo sapiens  |  Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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