Recombinant Human Excitatory amino acid transporter 1 (SLC1A3), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-KSI-tagged, 17.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Excitatory amino acid transporter 1 (SLC1A3), partial

Recombinant Human Excitatory amino acid transporter 1 (SLC1A3), partial — Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Purity >85%.

SKU: BCRECP-001645 Category:
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Product Specifications

Product SkuBCRECP-001645
Product DescriptionRecombinant Human Excitatory amino acid transporter 1 (SLC1A3) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 69-86aa. [Accession | P43003].
Uniprot No.P43003
Gene NamesSLC1A3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region69-86aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight17.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia
Disease Association: Episodic ataxia 6 (EA6) : A disorder characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Excitatory amino acid transporter 1 (SLC1A3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 69-86aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 17.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515, PubMed:7521911, PubMed:8123008). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Disease Association

Episodic ataxia 6 (EA6) : A disorder characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected in brain (PubMed:7521911, PubMed:8123008, PubMed:8218410). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911)

Subunit

Homotrimer (PubMed:28424515)

Gene: SLC1A3  |  Organism: Homo sapiens  |  Synonyms: Sodium-dependent glutamate/aspartate transporter 1; Solute carrier family 1 member 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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