Recombinant Human G-protein coupled bile acid receptor 1 (GPBAR1) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 36.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human G-protein coupled bile acid receptor 1 (GPBAR1) Protein

Recombinant Human G-protein coupled bile acid receptor 1 (GPBAR1) Protein — G protein-coupled receptor for bile acid. Purity >85%.

SKU: BCRECP-00296TM Category:
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Product Specifications

Uniprot No.Q8TDU6
Gene NamesGPBAR1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-330aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight36.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor for bile acid. Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum

Product Specifications

Recombinant Human G-protein coupled bile acid receptor 1 (GPBAR1) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-330aa, with N-terminal 10xHis-tagged tag, molecular weight 36.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for bile acid (PubMed:12419312, PubMed:12524422, PubMed:32698187, PubMed:32747649, PubMed:35858343). Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:12419312, PubMed:12524422, PubMed:32698187, PubMed:32747649, PubMed:35858343). GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed:12419312, PubMed:12524422, PubMed:32698187, PubMed:32747649, PubMed:35858343). Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order (PubMed:12524422, PubMed:32698187). Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation (PubMed:26541439). GPBAR1-mediated signaling promotes energy expenditure and adiposity reduction in brown adipose tissue by activating adenylate cyclase, leading to DIO2 activation (By similarity). Involved in bile acid promoted GLP-1 secretion (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum

Gene: GPBAR1  |  Organism: Homo sapiens  |  Synonyms: G protein-coupled receptor GPCR19; Membrane-type receptor for bile acids; hBG37
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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