Recombinant Human Malate dehydrogenase, Cytoplasmic (MDH1), partial, Homo sapiens (Human), E.coli, N-terminal GST-tagged, 63.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Malate dehydrogenase, Cytoplasmic (MDH1), partial

Recombinant Human Malate dehydrogenase, Cytoplasmic (MDH1), partial — Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Purity >90%.

SKU: BCRECP-001142 Categories: ,
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Product Specifications

Product SkuBCRECP-001142
Product DescriptionRecombinant Human Malate dehydrogenase, Cytoplasmic (MDH1) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 2-333aa. [Accession | P40925].
Uniprot No.P40925
Gene NamesMDHA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-333aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight63.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).

Biological Context

Subcellular Location: Cytoplasm, cytosol
Disease Association: Developmental and epileptic encephalopathy 88 (DEE88) : A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Malate dehydrogenase, Cytoplasmic (MDH1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-333aa, with N-terminal GST-tagged tag, molecular weight 63.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:2449162, PubMed:3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed:34012073)

Subcellular Location

Cytoplasm, cytosol

Disease Association

Developmental and epileptic encephalopathy 88 (DEE88) : A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homodimer

Gene: MDH1  |  Organism: Homo sapiens  |  Synonyms: Aromatic alpha-keto acid reductase; Cytosolic malate dehydrogenase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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