Recombinant Human Multifunctional methyltransferase subunit TRM112-like protein (TRMT112), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 30.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Multifunctional methyltransferase subunit TRM112-like protein (TRMT112)

Recombinant Human Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) — Acts as an activator of both rRNA/tRNA and protein methyltransferases. Purity >90%.

SKU: BCRECP-001857 Categories: ,
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Product Specifications

Product SkuBCRECP-001857
Product DescriptionRecombinant Human Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-125aa. [Accession | Q9UI30].
Uniprot No.Q9UI30
Gene NamesTRMT112
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-125aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight30.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA. The heterodimer with N6AMT1/HEMK2 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor.

Biological Context

Subcellular Location: Nucleus, nucleoplasm; Cytoplasm, perinuclear region

Product Specifications

Recombinant Human Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-125aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 30.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as an activator of both rRNA/tRNA and protein methyltransferases (PubMed:18539146, PubMed:20308323, PubMed:25851604, PubMed:31061526, PubMed:31328227, PubMed:31636962, PubMed:37283053). Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA (PubMed:25851604). The heterodimer with N6AMT1/HEMK2 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor (PubMed:18539146, PubMed:31061526, PubMed:31636962). The heterodimer with N6AMT1/HEMK2 also monomethylates 'Lys-12' of histone H4 (H4K12me1) (PubMed:31061526). The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species (PubMed:20308323). Together with methyltransferase THUMPD3, catalyzes the formation of N(2)-methylguanosine at position 6 in a broad range of tRNA substrates and at position 7 of tRNA(Trp) (PubMed:34669960, PubMed:37283053). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production (PubMed:25851604). Together with methyltransferase METTL5, specifically methylates the 6th position of adenine in position 1832 of 18S rRNA (PubMed:31328227, PubMed:33428944, PubMed:35033535, PubMed:37283053)

Subcellular Location

Nucleus, nucleoplasm; Cytoplasm, perinuclear region

Subunit

Part of the heterodimeric BUD23-TRM112 methyltransferase complex; this heterodimerization is necessary for the metabolic stability and activity of the catalytic subunit BUD23 (PubMed:25851604, PubMed:34948388). Part of the heterodimeric N6AMT1-TRM112 methyltransferase complex; this heterodimerization is necessary for S-adenosyl-L-methionine-binding to N6AMT1/HEMK2 (PubMed:20308323, PubMed:25851604, PubMed:31061526, PubMed:31632689, PubMed:31636962, PubMed:32969463, PubMed:34948388). Part of the heterodimeric ALKBH8-TRM112 methyltransferase complex (PubMed:20308323, PubMed:34948388). Part of the heterodimeric METTL5-TRM112 methyltransferase complex; this heterodimerization is necessary for the stability of the catalytic subunit METTL5 (PubMed:31328227, PubMed:32217665, PubMed:33428944, PubMed:34948388, PubMed:35033535, PubMed:37283053). Part of the heterodimeric THUMPD3-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit THUMPD3 (PubMed:34669960, PubMed:34948388, PubMed:37283053). Part of the heterodimeric THUMPD2-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit THUMPD2 (PubMed:34948388, PubMed:37283053). Part of the heterodimeric TRMT11-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit TRMT11 (PubMed:34948388, PubMed:37283053)

Gene: TRMT112  |  Organism: Homo sapiens  |  Synonyms: tRNA methyltransferase 112 homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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